@article {329, title = {Genetic background influences the effect of thirdhand smoke exposure on anxiety and memory in Collaborative Cross mice.}, journal = {Sci Rep}, volume = {11}, year = {2021}, month = {2021 06 24}, pages = {13285}, abstract = {

Growing evidence indicates that thirdhand smoke (THS) exposure induces many adverse health effects. However, it is unclear how THS exposure affects behavior and how host genetic background modulates phenotypic changes. Here we used the Collaborative Cross (CC) mouse population-based model to assess behavioral alterations immediately after THS exposure from 4 to 9\ weeks of age. We first measured anxiety-like behavior in six strains using light/dark box combined with a custom multivariate mouse tracking system. We developed an anxiety risk scoring system based on anxiety-related traits and then evaluated the THS impact on them. THS exposure significantly decreased anxiety risk in CC019 (P = 0.002) and CC051 (P = 0.009), but increased anxiety risk in CC036 (P \< 0.001), while the other three strains did not show significant changes in anxiety-related traits. Such differences were driven by female mice for the six measures of anxiety-like behavior. Memory potential was measured in the same cohort of mice using the passive avoidance assay. Both THS-exposed male and female CC019 mice displayed significant memory loss compared to controls while no significant changes were found in the other five strains. This study provides strong evidence that THS exposure leads to strain-dependent changes in anxiety-like behavior and memory, suggesting that host genetic variations play a critical role in individual susceptibility to THS-induced effects.

}, keywords = {Animals, Anxiety, Collaborative Cross Mice, Female, Humans, Male, Memory, Risk Factors, Tobacco Smoke Pollution}, issn = {2045-2322}, doi = {10.1038/s41598-021-92702-1}, author = {He, Li and Wang, Pin and Schick, Suzyann F and Huang, Abel and Jacob, Peyton and Yang, Xu and Xia, Yankai and Snijders, Antoine M and Mao, Jian-Hua and Chang, Hang and Hang, Bo} } @article {328, title = {Thirdhand cigarette smoke leads to age-dependent and persistent alterations in the cecal microbiome of mice.}, journal = {Microbiologyopen}, volume = {10}, year = {2021}, month = {2021 06}, pages = {e1198}, abstract = {

The gut microbiome composition is influenced by many factors including environmental exposures. Here, we investigated the effect of thirdhand cigarette smoke (THS) and exposure age on gut microbiome diversity. C57BL/6 mice were exposed to THS at human exposure relevant levels for three weeks during three different life stages: postnatal (0-3\ weeks of age), pubescent (4-7\ weeks of age), and adult (9-12\ weeks of age), respectively. Cecal microbiome profiles were assessed at 17\ weeks of age by 16S rRNA gene sequencing. We found that age at THS exposure strongly influenced the cecal microbiome composition. Although postnatal THS exposure significantly influenced the microbial composition, pubescent and adulthood exposures only had minor effects. The microbiome of postnatally THS-exposed mice significantly increased several degradation pathways that regulate glycolysis and pyruvate decarboxylation, and significantly decreased coenzyme A biosynthesis and pyrimidine deoxyribonucleoside salvage. Our results indicate that mouse postnatal development is particularly susceptible to persistent THS exposure effects on the gut microbiome.

}, keywords = {Age Factors, Animals, Bacteria, Cecum, Environmental Exposure, Female, Gastrointestinal Microbiome, Humans, Male, Mice, Mice, Inbred C57BL, Smoke, Tobacco, Tobacco Smoke Pollution}, issn = {2045-8827}, doi = {10.1002/mbo3.1198}, author = {He, Li and Zhou, Yan-Xia and Zhang, Yuqing and Hang, Bo and Chang, Hang and Schick, Suzaynn F and Celniker, Susan E and Xia, Yankai and Snijders, Antoine M and Mao, Jian-Hua} } @article {322, title = {Identification of a novel 15-gene expression signature predicting overall survival of human colorectal cancer.}, journal = {Clin Transl Med}, volume = {10}, year = {2020}, month = {2020 Dec}, pages = {e258}, issn = {2001-1326}, doi = {10.1002/ctm2.258}, author = {Jiang, Chengfei and Zhao, Yue and Yuan, Binbin and Chang, Hang and Hang, Bo and Snijders, Antoine M and Mao, Jian-Hua and Zou, Xiaoping and Wang, Pin} } @article {307, title = {Systematic Analysis of Impact of Sampling Regions and Storage Methods on Fecal Gut Microbiome and Metabolome Profiles.}, journal = {mSphere}, volume = {5}, year = {2020}, month = {2020 01 08}, abstract = {

The contribution of human gastrointestinal (GI) microbiota and metabolites to host health has recently become much clearer. However, many confounding factors can influence the accuracy of gut microbiome and metabolome studies, resulting in inconsistencies in published results. In this study, we systematically investigated the effects of fecal sampling regions and storage and retrieval conditions on gut microbiome and metabolite profiles from three healthy children. Our analysis indicated that compared to homogenized and snap-frozen samples (standard control [SC]), different sampling regions did not affect microbial community alpha diversity, while a total of 22 of 176 identified metabolites varied significantly across different sampling regions. In contrast, storage conditions significantly influenced the microbiome and metabolome. Short-term room temperature storage had a minimal effect on the microbiome and metabolome profiles. Sample storage in RNALater showed a significant level of variation in both microbiome and metabolome profiles, independent of the storage or retrieval conditions. The effect of RNALater on the metabolome was stronger than the effect on the microbiome, and individual variability between study participants outweighed the effect of RNALater on the microbiome. We conclude that homogenizing stool samples was critical for metabolomic analysis but not necessary for microbiome analysis. Short-term room temperature storage had a minimal effect on the microbiome and metabolome profiles and is recommended for short-term fecal sample storage. In addition, our study indicates that the use of RNALater as a storage medium of stool samples for microbial and metabolomic analyses is not recommended. The gastrointestinal microbiome and metabolome can provide a new angle to understand the development of health and disease. Stool samples are most frequently used for large-scale cohort studies. Standardized procedures for stool sample handling and storage can be a determining factor for performing microbiome or metabolome studies. In this study, we focused on the effects of stool sampling regions and stool sample storage conditions on variations in the gut microbiome composition and metabolome profile.

}, issn = {2379-5042}, doi = {10.1128/mSphere.00763-19}, author = {Liang, Yali and Dong, Tianyu and Chen, Minjian and He, Lianping and Wang, Tingzhang and Liu, Xingyin and Hang Chang and Mao, Jian-Hua and Hang, Bo and Snijders, Antoine M and Xia, Yankai} } @article {308, title = {Thirdhand smoke: Genotoxicity and carcinogenic potential.}, journal = {Chronic Dis Transl Med}, volume = {6}, year = {2020}, month = {2020 Mar}, pages = {27-34}, abstract = {

Thirdhand smoke (THS), the residual tobacco smoke remaining in the environment after tobacco has been smoked, represents a hidden and underestimated public health hazard. Evidence supports its widespread presence in indoor environments. Exposure to secondhand smoke (SHS), a precursor of THS, has been well documented as a risk factor for human cancers, especially lung cancer. However, the concept of THS as a distinct entity that poses health risks for small children has developed only recently and the associations of THS with cancer risk and other chronic diseases are poorly understood due to limited numbers of studies to date. In this perspective, we mainly summarize all published studies on the genotoxicity and carcinogenic potential of THS exposure. These studies begin to fill the knowledge gap in our understanding of cancer risk of THS. Accumulating data from existing and future studies will help reduce the tobacco-related cancer incidence through changes in lifestyle and tobacco control policies.

}, issn = {2589-0514}, doi = {10.1016/j.cdtm.2019.08.002}, author = {Hang, Bo and Wang, Pin and Zhao, Yue and Hang Chang and Mao, Jian-Hua and Snijders, Antoine M} }