@article {326,
	title = {A new platform for ultra-high dose rate radiobiological research using the BELLA PW laser proton beamline.},
	journal = {Sci Rep},
	volume = {12},
	year = {2022},
	month = {2022 01 27},
	pages = {1484},
	abstract = {<p>Radiotherapy is the current standard of care for more than 50\% of all cancer patients. Improvements in radiotherapy (RT) technology have increased tumor targeting and normal tissue sparing. Radiations at ultra-high dose rates required for FLASH-RT effects have sparked interest in potentially providing additional differential therapeutic benefits. We present a new experimental platform that is the first one to deliver petawatt laser-driven proton pulses of 2\&nbsp;MeV energy at 0.2\&nbsp;Hz repetition rate by means of a compact, tunable active plasma lens beamline to biological samples. Cell monolayers grown over a 10\&nbsp;mm diameter field were exposed to clinically relevant proton doses ranging from 7 to 35\&nbsp;Gy at ultra-high instantaneous dose rates of 10\&nbsp;Gy/s. Dose-dependent cell survival measurements of human normal and tumor cells exposed to LD protons showed significantly higher cell survival of normal-cells compared to tumor-cells for total doses of 7\&nbsp;Gy and higher, which was not observed to the same extent for X-ray reference irradiations at clinical dose rates. These findings provide preliminary evidence that compact LD proton sources enable a new and promising platform for investigating the physical, chemical and biological mechanisms underlying the FLASH effect.</p>
},
	keywords = {Cell Line, Humans, Lasers, Monte Carlo Method, Neoplasms, Proton Therapy, Radiation Oncology, Radiobiology, Radiometry, Radiotherapy Dosage, Synchrotrons},
	issn = {2045-2322},
	doi = {10.1038/s41598-022-05181-3},
	author = {Bin, Jianhui and Obst-Huebl, Lieselotte and Mao, Jian-Hua and Nakamura, Kei and Geulig, Laura D and Chang, Hang and Ji, Qing and He, Li and De Chant, Jared and Kober, Zachary and Gonsalves, Anthony J and Bulanov, Stepan and Celniker, Susan E and Schroeder, Carl B and Geddes, Cameron G R and Esarey, Eric and Simmons, Blake A and Schenkel, Thomas and Blakely, Eleanor A and Steinke, Sven and Snijders, Antoine M}
}
@article {328,
	title = {Thirdhand cigarette smoke leads to age-dependent and persistent alterations in the cecal microbiome of mice.},
	journal = {Microbiologyopen},
	volume = {10},
	year = {2021},
	month = {2021 06},
	pages = {e1198},
	abstract = {<p>The gut microbiome composition is influenced by many factors including environmental exposures. Here, we investigated the effect of thirdhand cigarette smoke (THS) and exposure age on gut microbiome diversity. C57BL/6 mice were exposed to THS at human exposure relevant levels for three weeks during three different life stages: postnatal (0-3\&nbsp;weeks of age), pubescent (4-7\&nbsp;weeks of age), and adult (9-12\&nbsp;weeks of age), respectively. Cecal microbiome profiles were assessed at 17\&nbsp;weeks of age by 16S rRNA gene sequencing. We found that age at THS exposure strongly influenced the cecal microbiome composition. Although postnatal THS exposure significantly influenced the microbial composition, pubescent and adulthood exposures only had minor effects. The microbiome of postnatally THS-exposed mice significantly increased several degradation pathways that regulate glycolysis and pyruvate decarboxylation, and significantly decreased coenzyme A biosynthesis and pyrimidine deoxyribonucleoside salvage. Our results indicate that mouse postnatal development is particularly susceptible to persistent THS exposure effects on the gut microbiome.</p>
},
	keywords = {Age Factors, Animals, Bacteria, Cecum, Environmental Exposure, Female, Gastrointestinal Microbiome, Humans, Male, Mice, Mice, Inbred C57BL, Smoke, Tobacco, Tobacco Smoke Pollution},
	issn = {2045-8827},
	doi = {10.1002/mbo3.1198},
	author = {He, Li and Zhou, Yan-Xia and Zhang, Yuqing and Hang, Bo and Chang, Hang and Schick, Suzaynn F and Celniker, Susan E and Xia, Yankai and Snijders, Antoine M and Mao, Jian-Hua}
}
