@article {194,
	title = {Mass spectrometry imaging for in situ kinetic histochemistry.},
	journal = {Sci Rep},
	volume = {3},
	year = {2013},
	month = {2013},
	pages = {1656},
	abstract = {<p>Tissues are composed of diverse cell subpopulations each with distinct metabolic characteristics that influence overall behavior. Unfortunately, traditional histopathology imaging techniques are {\textquoteright}blind{\textquoteright} to the spatially ordered metabolic dynamics within tissue. While mass spectrometry imaging enables spatial mapping of molecular composition, resulting images are only a static snapshot in time of molecules involved in highly dynamic processes; kinetic information of flux through metabolic pathways is lacking. To address this limitation, we developed kinetic mass spectrometry imaging (kMSI), a novel technique integrating soft desorption/ionization mass spectrometry with clinically accepted in vivo metabolic labeling of tissue with deuterium to generate images of kinetic information of biological processes. Applied to a tumor, kMSI revealed heterogeneous spatial distributions of newly synthesized versus pre-existing lipids, with altered lipid synthesis patterns distinguishing region-specific intratumor subpopulations. Images also enabled identification and correlation of metabolic activity of specific lipids found in tumor regions of varying grade.</p>},
	keywords = {Cell Line, Equipment Design, Equipment Failure Analysis, Experimental, Humans, Lipids, Mass Spectrometry, Molecular Imaging, Neoplasms, Tumor},
	issn = {2045-2322},
	doi = {10.1038/srep01656},
	author = {Louie, Katherine B and Bowen, Benjamin P and McAlhany, Stephanie and Huang, Yurong and Price, John C and Jiang-Hua Mao and Hellerstein, Marc and Northen, Trent R}
}
